11. The DNA molecule below is incubated with all four dNTPs, Mg+ and a DNA polymerase…

11. The DNA molecule below is incubated with all four dNTPs, Mg+ and a DNA polymerase that lacks 5’+3′ exonuclease activity but has 3-5’exonuclease activity and polymerase activity. What will be the structure of the final product and what is the first step in the reaction sequence? 3′ OH-TGCGAATTAGCGACAT-P5′ 5’P-ATCGGTACGGCGCTTA-P 3′ 12. You are screening a chemical for potential mutagenicity using the Ames test. You used a his-S. typhimurium bacterium for this test and obtained the following results: Chemical + Control (HO) Chemical rat liver extract 317 20 A) What was the purpose of also testing water and why did it result in 21 colonies? B) The rat liver appears to modify the chemical into a mutagen. How would you show that this is due to modification the chemical and not due to rat liver components? 4. In Drosophila kidney bean eye (k), cardinal eye (c), and ebony body (e) are recessive alleles. The respective wild type alleles are k+, ct and et. If homozygous kidney, cardinal females are mated to homozygous ebony males (P generation), the F1 progeny are all wild- type. If heterozygous F1 females are mated with kidney, cardinal, ebony males, the following progeny are produced: k, c, et k+, c+, e k, c+, e k+, c, e+ k+, c, e k, c+, et k, c, e k+, c+, et Are any of the genes linked? If so, show the map of the linked genes?