Fall 2017 CHE 380: Introduction to Biotechnology Exam3 (I hr 20 min) Question 3 (28 polnts)…

Fall 2017 CHE 380: Introduction to Biotechnology Exam3 (I hr 20 min) Question 3 (28 polnts) and label the reaction was conducted on the b. Read the sequence A DNA sequencing was template strand in a thin capillary gel tube containing primers, DNA polymerase, deox yribuneeleotides and|メイenmcaT luorescently tagged dideoxyribunecleotides and a e. The smallest fragment is computer was used to record the fluorescent peaks during fluorescently tagged with (A)ddATP (B) ddGTP (C) ddCTP (D) ddTTP was used to reoribunecleotidesnanTCAATGaTa Crara the reaction (the Genetic Code is on page 4 d. Determine the unknown template sequence and label the ends Determine the mRNA transcript Determine the amino acid chain f. produced from the DNA 10 15 Time (s) E. Determinea four base pair restriction site on the DNA a. The largest fragment is fluorescently tagged with (A)ddATP (B) ddGTP (C) ddCTP (D) ddTTP