One method of analyzing amino acids is the van Slyke method. The characteristic amino groups (-NH2 )

One method of analyzing amino acids is the van Slyke method.
The characteristic amino groups (_NH2 ) in protein material are allowed
to react with nitrous acid, HNO2 , to form N2 gas. From the volume of the gas,
the amount of amino acid can be determined. A 0.0604-g sample of a biological
sample containing glycine, CH2 (NH2 )COOH, was analyzed by the van Slyke method
and yielded 3.70 mL of N2 collected over water at a pressure of 735 torr and 29
°C. What was the percentage of glycine in the sample? CH2 _ _NH2 _ _CO2 H + HNO2 _ CH2 (OH)CO2 H + H2 O
+ N2